In vitro regeneration of triploid plants from mature endosperm culture of commercial passionfruit (Passiflora edulis Sims)
- Otoni, Wagner Campos
- Antoniazzi, Camila Aparecida
- Faria, Rodrigo Brito de
- Carvalho, Paula Pinheiro de
- Mikovski, Andreia Izabel
- Carvalho, Ilio Fealho de
- Matos, Elyabe Monteiro de
- Reis, Aryane Campos
- Viccin, Lyderson Facio
- Pinto, Daniela Lopes Paim
- Rocha, Diego Ismael
- Silva, Maurecilne Lemes da
DOIAbstract
Due to the triploid nature of endosperm, an embryonic reserve tissue, in vitro culture of endosperm tissues has been considered a direct method for production of polyploids. In the present study, we report the establishment of an in vitro regeneration system from endosperm culture for production of triploid Passiflora edulis plants, the main commercial species of passionfruit. Surface-sterilized endosperms were cultured in MS medium with different concentrations of benzyladenine (BA), thidiazuron (TDZ), and kinetin (KIN). The cultures were maintained in a growth chamber under controlled conditions, for 60 days. Thidiazuron was the only type of cytokinin that induced shoot production in the endosperm tissues; the highest number of shoots was...
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