The Mediterranean Forecasting System (MFS) has been operational for a decade, and is continuously providing forecasts and analyses for the region. These forecasts comprise local- and basin-scale information of the environmental state of the sea and can be useful for tracking oil spills and supporting search-and-rescue missions. Data assimilation is a widely used method to improve the forecast skill of operational models and, in this study, the three-dimensional variational (OceanVar) scheme has been extended to include Argo float trajectories, with the objective of constraining and ameliorating the numerical output primarily in terms of the intermediate velocity fields at 350 m depth. When adding new datasets, it is furthermore crucial to e...