A high-throughput platform for detailed lipidomic analysis of a range of mouse and human tissues
- Furse, Samuel
- Fernandez-Twinn, Denise S.
- Jenkins, Benjamin
- Meek, Claire L.
- Williams, Huw E. L.
- Smith, Gordon C. S.
- Charnock-Jones, D. Stephen
- Ozanne, Susan E.
- Koulman, Albert
DOIAbstract
Abstract: Lipidomics is of increasing interest in studies of biological systems. However, high-throughput data collection and processing remains non-trivial, making assessment of phenotypes difficult. We describe a platform for surveying the lipid fraction for a range of tissues. These techniques are demonstrated on a set of seven different tissues (serum, brain, heart, kidney, adipose, liver, and vastus lateralis muscle) from post-weaning mouse dams that were either obese (> 12 g fat mass) or lean (<5 g fat mass). This showed that the lipid metabolism in some tissues is affected more by obesity than others. Analysis of human serum (healthy non-pregnant women and pregnant women at 28 weeks’ gestation) showed that the abundance of several ph...
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