Preparation of Monoclonal Antibody against Rebaudioside A and Its Immunoassay Application

甜菊（Steviarebaudiana(Bertoni)Hemsl.）是一种具有商业前景的甜味剂。甜菊苷(Stevioside，ST)和莱包迪苷A（RebaudiosideA，RA）是甜菊提取物中最主要的两种物质，其中RA具有许多的优点，譬如口感好，甜度高，稳定性强，无毒副作用等。因此RA更加适合用于食品饮料药物中。 本研究的目的就是通过制备莱包迪苷A的单克隆抗体，从而尝试建立以此为基础的酶联免疫测定（Enzyme-linkedimmunosorbentassay，ELISA）方法，对甜菊叶中的RA进行定量分析。 首先采用高碘酸钠法合成人工抗原RA-HSA、RA-OVA。通过MAIDI-T...Stevia （Stevia rebaudiana (Bertoni) Hemsl.）is a commercially promising high-quality sweetener. Stevioside (ST) and Rebaudioside A (RA) are the two main compounds from the stevia. Between them, RA has a lot of advantages, such as good taste, high sweet potential, high steadily, no side-effect, ect. All those characteristics make RA a better sweetener suitable for food industry comparing with others...学位：理学硕士院系专业：生命科学学院_生物化学与分子生物学学号：2162007115195