Preparation of Monoclonal Antibody against Stevioside and Development of ELISA Method for Stevioside

甜菊苷(stevioside，ST)为甜叶菊中所含的主要甜味成分，是理想的高甜度、低热量，对糖尿病和高血糖脂有很好疗效的一种新型高安全性甜味剂，也可作为一些药品的品位改良剂和矫味剂，从而广泛应用于食品和药物。目前，甜菊叶中甜菊苷的提取工艺已经日趋成熟，然而甜菊苷的准确含量的检测方法主要是使用HPLC，还存在不足之处，因此，建立简单，快速，准确的甜菊苷定量方法对于甜菊苷的生产和应用都具有极其重要的意义。 本研究利用高碘酸纳法将甜菊苷（ST）分别与人血清白蛋白（HSA）和牛血清白蛋白（BSA）进行偶联，成功制备了免疫抗原和包被抗原，经MALDI-TOFMS鉴定得HSA，BSA上分别挂13，8个S...Stevioside, as the major sweet ingredient of Stevia rebaudiana, is a new kind of natural high sweetness, low-calorie and high safety sweetener, which has physiological functions including anti-hyperglycemia and anti-diabetes. It’s also can be used as improving the tastes of medicine. Its stable chemical and physical properties and non-fermentability lead to long shelf life and widely using in food...学位：理学硕士院系专业：生命科学学院_生物化学与分子生物学学号：2162007115201