Application of Real-time PCR Technology on the Detection of Pathogenic Bacteria of Septicemia

血液中感染微生物，称之为血流感染（BSI）;当微生物在血液中能够生长繁殖，并且产生毒素，引起全身性感染时，称之为败血症(Septicemia)。近年来，败血症发病率和死亡率逐年增长，已严重威胁人类生命健康，检测败血症中感染的病原体种类对其治疗至关重要。本论文利用实时PCR技术围绕败血症病原体诊断展开了研究，以建立一种快速、准确、成本低、操作简便的微生物鉴定方法。我们使用媒介探针技术结合探针熔解曲线法，建立了适合临床快速鉴定败血症病原体种类的检测体系。 第一章，介绍了败血症的背景、临床表现、发病诱因，病原学变迁、抗菌治疗，然后比较了常见的败血症诊断方法及其优缺点，包括血培养及生化鉴定、PCR检...Microbial infection in the blood is known as bloodstream infection (BSI). When the microorganisms are able to thrive in the blood, and produce toxins that cause systematic infection are known as sepsis (Septicemia). In recent years, the incidence and mortality of sepsis have increased year by year, which has seriously threatened the health of human life. In this paper, a real-time PCR method was u...学位：理学硕士院系专业：生命科学学院_细胞生物学学号：2162014115250