Telomerase activity in gastric cancer and other gastric diseases and its clinical implication

目的 :研究端粒酶在胃癌及其它胃疾病中的表达情况 ,以评价其临床诊断意义。方法 :采用TRAP法检测 32例胃癌 ,35例胃炎 ,8例肠上皮化生 ,8例胃溃疡 ,2例正常胃粘膜端粒酶活性。结果 :72 % (2 3/ 32 )胃癌组织 ,71% (2 5 / 35 )胃炎组织端粒酶呈阳性。肠上皮化生端粒酶呈阳性率为 5 / 8。 8例胃溃疡皆为端粒酶阴性。结论 :由于大多胃炎组织 (71% )端粒酶呈阳性 ,这将影响端粒酶在胃癌临床诊断中的应用。Objective:To detect telomerase activity in gastric cancer and other gastric diseases for the evaluation of its clinical implication Method:TRAP method was used to determine telomerase activity in 32 cases of gastric cancer,35 cases of gastritis,8 cases of intestinal metaplasia,8 cases of gastric ulcer, and 2 cases of normal gastric mucosa Results:Telomerase activity was positive in 72%(23/32) of gastric cancer samples, 71%(25/35) of gastritis tissues, and of 5 out of 8 biopsies of intestinal metaplasia No telomerase activity was detected in 8 cases of gastric ulcer Conclusion:Telomerase activity is unsuitable for clinical diagnosis of gastric cancer because it is also detected in majority of tissues of gastritis福建省卫生厅基