Effect of Naphthalene Intimidation on Seedling Germination and Antioxidase in Avicennia marina

采用砂基栽培,研究污染物多环芳烃萘(Nap)不同暴污强度( 0、20、40、60、80、100mg/L)和暴污时间的环境胁迫下,对红树植物白骨壤(Avicenniamarina)幼苗萌发初期的萌生及子叶超氧化物歧化酶(SOD)、过氧化物酶(POD)活性和膜脂质过氧化作用的影响.结果表明:Nap污染胁迫对红树白骨壤幼苗的萌生,随着暴污强度和暴污时间的增加而影响愈加明显;幼苗萌发初期抗Nap胁迫生长的浓度范围在40mg/L以下.在暴污的较短时间内,幼苗子叶SOD活性有着积极的响应;随着暴污时间的延长,SOD活性则逐步受到抑制,达30d后酶活性略普遍低于对照.受Nap的胁迫,幼苗子叶POD活性提高;POD有效防御Nap胁迫的浓度范围在60mg/L以下.幼苗子叶中抗氧化防御系统,在萌发初期对有效防御膜脂质过氧化的Nap胁迫浓度在60mg/L以下.结果表明,白骨壤幼苗的萌发初期,对Nap污染的环境胁迫有较强的抗性能力.This paper mainly dealt with some physiological responses of mangrove species A. marina seedling to the pollution of a series of different Nap concentration (0,20,40,60,80,100 mg/L) in sand culture,such as the variance of SOD and POD activities,and Lipid peroxidation during the seedling germination.The results show that the germination of A.marina was obviously influenced by Nap pollution and this influence became more evident with the gradual increasing of Nap concentration and treatment time; less than 40 mg/L of Nap concentration is the adaptive bound during the early seedling germination period.Under the Nap pollution during the earlier time the SOD activity of seedling cotyledons showed positive response,but with the time went on,the SOD activity was inhibited and that of 30 days was universally lower than 15 days.Under the Nap intimidation,the POD activity of seedling cotyledons rose and the efficacious recovery only occurred in the condition that Nap concentration was under 60 mg/L.For the antioxidase system of seedling cotyledons,the effective recovery to lipid peroxidation during the seedling germination happened under the situation of less than 60 mg/L Nap concentration.As a result,during the early germination period,A.marina seedling has stronger resistibility to the Nap pollution