CO-CULTURE OF RED TIDE ALGAE ALEXANDRIUM TAMARENSE (LEBOUR) BALECH WITH BACTERIA AND ITS MICRO-ECOLOGICAL EFFECTS

为研究赤潮藻类与海洋细菌的微生态关系 ,在实验室模拟条件下将塔马亚历山大藻 (Alexandriumtamarense (Lebour)Balech)与海底沉积物中分离的两种细菌 (Bacillusmegaterium ,S7;B .halmapulus,S1 0 )共同培养 ,并探讨了藻菌共培养对A .tamarense生长以及对水体中胞外酶 β 葡萄糖苷酶活性 ( β GlcA)的影响 .实验结果表明 ,在培养的第 2周内 ,与细菌S7或S1 0 共培养的A .tamarense细胞数分别比CK增加约 12 %和 2 4% ,而在第 3周内则各增加 2 3%和 5 %左右 .另外 ,分别与A .tamarense共培养的细菌S7或S1 0 的 β GlcA变化趋势相似 ,呈马鞍型曲线 .在A .tamarense生长的后期 ,β GlcA随着藻细胞的裂解程度的增加而迅速提高 .图 3参 25One of red tide algae, Alexandrium tamarense (Lebour) Balech,was co-cultured with two species of bacteria, Bacillus megaterium (S 7) and B. halmapulus (S 10) isolated from Xiamen western sea, to investigate the micro-ecological relationship between the red tidal algae and sea bacteria. The results showed that the cell number of the alga A. tamarense increased by about 12% and 24% in the second week, and 23% and 5% in the third week compared with the control. Moreover, there was a roughly similar variation trend of the activity of extra-cellular enzyme and β-glucosidase in the growth media of bacteria S 7 or S 10 co-cultured with the alga. The β-glucosidase activity rapidly increased at the later stage of algal growth accompanying the increase of the decay of algal cells. Tab 3, Ref 25国家自然科学基金资助项目 (九五重大基金项目 :39790 1 1 0 ,面上基金项目 :30 0 70 1 57)~