The Electron Microscopic in situ Hybridization and Its Application

将原位分子杂交技术应用于电镜水平 ,观察标记的特定DNA、mRNA和RHA在细胞和 或组织内的超微结构定位的方法 ,称为电镜原位分子杂交技术。本文综述了电镜原位分子杂交技术的建立及其分类 ,并详细介绍了非放射性电镜原位分子杂交技术的基本操作程序及注意事项 ,最后对电镜原位分子杂交技术的应用做了简要介绍。The technique of electron microsco pic \%in situ\% hybridization is applying \%in situ\% hybridization at the electron microscopic level. It is mainly used in the ultrastructural localization of the labled DNA、RNA and RHA in a cell and/or a tissue. In this paper I mainly elaborated its establishment and classification, and the operation procedure of nonradioactive electron microscopic \%in situ \%hybridization and some points for attention. In the end I also discussed its application for research.国家自然科学基金资助 (No .39870 46 1)~