Screening and characterization of cellulase-producing Penicillium sp.T24-2

从稻田腐烂秸秆中分离到一批纤维素分解菌株。通过滤纸崩解测试、刚果红纤维素平板识别,以及产酶鉴定,筛选得到一株分解纤维素能力较强的真菌。经形态观察和18S r DNA基因片断分析,鉴定该菌株为青霉。对菌株的液态发酵条件进行研究,该菌株培养基含3%稻草粉、0.25%尿素和无机盐营养液,最佳产酶条件为:自然pH,30℃,130r/min发酵4d。该菌株的CMC酶活和滤纸酶活最高分别达到45.01I U/mL和6.89I U/mL。随后对该菌酶解稻草粉进行研究,糖化率达到40.17%。研究表明,青霉T24-2菌株在秸秆综合利用上具有良好的应用前景。A high cellulase-producing strain was isolated from putrid straw.It was identified as Penicillium sp.by its biological property and analysis of 18S rDNA sequences.The liquid fermentation conditions for cellulase-producing were studied extensively.The optimum conditions for cellulase production were obtained as:3% straw powder as carbon source,0.2% urea as nitrogen source,fermentation at 30 ℃ and natural pH for 4d.The maximum activities of CMCase and FPA were obtained as 45.01 IU/mL and 6.89 IU/mL,respectively.The reducing sugar yield of saccharification reached to 40.2%.The research results suggested a perspective in the utilization of straw resource.国家863计划项目(2001AA515030);; 福建省科技重点项目(2005I016);; 厦门大学新世纪优秀人才支持计划资