Isolation and Genetic Analysis for Rice Mutants Treated with ~(60)Co γ-Ray

为了研究水稻发育的分子机制,我们利用γ射线对粳稻品种9522进行诱变,共诱变了3 000 g种子,M1代单株收种,M2代移栽5 963个株系于上海农科院.在M2代中筛选突变体,并在M3代中复选,最终得到了叶、株型育性等各类形态突变体666份.对其中162株突变体进行了遗传分析,有53.61%的突变体出现3∶1的分离,这些突变体是隐性的单位点突变.同时,这些突变体的分离也为水稻功能基因分离和功能鉴定等研究打下了良好的基础,并为水稻发育的研究提供了宝贵的材料.3,000 gram seeds of japonica cultivar 9522 background were treated with 60Co γ-Ray for the study on the molecular mechanism of rice development.Then 5 963 lines of M2 progeny were transplanted in the paddy field of Shanghai Agriculture Academy.666 mutants of the M3 progeny showed abnormal development in several organs such as flower leaf and seed,among which,162 mutants were selected randomly for further genetic analysis,53.61 percent of which showed a good fit to the expected ration of 3∶1.The result indicated that a single recessive nuclear gene might control these mutant phenotype.Isolation and genetic analysis for these mutants would pave the way for further cloning of important gene in rice and provide valuable materials for the study of rice development.国家科技部国家重点基础研究发展项目(2005CB120802)资