Typing the genes of hepatitis C virus by reverse dot blot

目的:分析厦门地区及肝病患者不同临床类型丙型肝炎病毒(HCV)感染株的基因型。方法:用反向点杂交技术(RDB)对109例HCVRNA阳性的血清进行基因分型。结果:109例HCVRNA阳性血清中,其中单一型感染者98例(89.91%),混合型感染11例(10.09%),单一型感染者中以1b型占绝对优势,共90例(82.57%),2a/2c及6a型分别为2例和3例,另有3例1b+2a/2c和8例1b+6a混合型;HCV1b型在急、慢性肝炎、肝硬化及肝癌患者中均占绝对优势,其余有少量2a/2c、6a及某些混合感染型。结论:厦门地区HCV感染者中,以HCV1b基因型为主,其次为HCV6a,另有少数2a/2c型。不同基因型在不同临床类型中的分布无明显差异。Objective To analyze the genotypes of isolates of HCV from different clinical styles of liver disease in the Xiamen area. Methods 109 cases of HCV-RNA serum samples were typed by reverse dot blot (RDB). Results Of 109 cases,98 were single genotype (89.91%) and 11 were multi-genotypes (10.09%). There were 90 cases of HCV-1b (82.57%),2 HCV-2a/2c and 3 HCV-6a in the single genotype and 3 HCV genotype 1b+2a/2c,and 8 HCV genotype 1b+6a in the hybrid genotype. The isolates of HCV-1b were prominent in acute hepatitis,chronic hepatitis,hepatocirrhosis and hepatocellular carcinoma and the remaining was the isolates of HCV-2a/2c,HCV-6a and other genotypes. Conclusions HCV-1b is the main genotype in the individuals infected by HCV in the Xiamen area and followed by HCV-6a.A few infected individuals are HCV-2a/2c. There is no significant difference in the distribution of HCV genotypes in various types of liver disease