Effects of tributyltin on alkaline phosphatase,aaciphosphatase andadenosine triphosphatase gill activity of digestive gland in clam Meretrix meretrix

在实验生态条件下,观察浓度分别为0.1、1.0和10ng/L的三丁基锡(TBT)暴露2、8、20d以及恢复7d和20d后对文蛤鳃酸性磷酸酶(aaciphosphatase,ACP)、碱性磷酸酶(alkalinephosphatase,AKP)以及Na+,K+-ATPase活性的影响。结果显示在暴露早期TBT不影响ACP、AKP活性,暴露时间延长则主要表现出诱导作用。TBT具有抑制Na+、K+-ATPase活性作用,可观察效应浓度为0.1ng/L。Na+、K+-ATPase可作为一种潜在的有机锡污染监测的生物标志物。The effects on alkaline phosphatase, aaciphosphatase and Na~+K~+-ATPase activities in the gill of Meretrix meretrix, which was exposed to tributyltin (TBT) at the environmental levels (0.1ng/L, 1.0ng/L, 10ng/L as Sn) for 2, 8, 20 days and recovered for 7, 20 days, were evaluated. The results showed that the activities of alkaline phosphatase, aaciphosphatase were no effect at earlier exposure period, and were principally inducted with prolonged exposure time. The activities of Na~+,K~+-ATPase in Meretrix meretrix were inhibited after exposure to TBT. The lowest observable effect concentration for Na~+K~+-ATP were 0.1 ng /L. Na~+,K~+-ATPase could be a potential biomarkers to monitor the organotins pollution.厦门大学细胞生物学与肿瘤细胞工程教育部重点实验室开放基金资助(2004109