Determination of Tetracyclines by a New Spectrum Technique

四环素类抗生素属于弱荧光物质,不能在荧光计上直接检测,一般需要经过衍生反应。为了能在荧光计上实现了四环素类抗生素的直接检测,必须采用新的光谱技术,使荧光计达到了一机两用的功能。实验结果表明四环素类抗生素的线性范围均为0.10~9.00μg.mL-1,四环素、土霉素、金霉素和多西环素的检测限分别为0.065,0.067,0.068和0.070μg.mL-1。Tetracyclines are light-fluorescence substances, which cannot be detected directly by fluorometry. Herein a new spectrum method was proposed to detect tetracyclines directly by fluorometry. Under optimal conditions, the calibration graph is linear over the range 0.10-9.00 μg·mL -1 for tetracyclines, and the detection limits of tetracycline, oxytetracycline, chlortetracycline and doxycycline are 0.065, 0.067, 0.068 and 0.070 μg·mL -1 , respectively.福建省自然科学基金(D0310005)资助项