Study on the Genetic Structure of the Wild Stock of Hapalogenys mucronatus in Xiamen Bay

采用RAPD和ISSR分子标记技术对厦门近海横带髭鲷野生群体46个个体进行遗传多样性分析.在RAPD分析中筛选出31个随机引物,共扩增产生235个可统计的条带,其中多态性条带106个,多态位点百分率为45.11%,Shan-non′s遗传多样性指数为0.243 0;在ISSR分析中筛选出16个ISSR引物,共扩增产生119个可统计的条带,其中多态性条带76个,多态位点百分率为63.78%,Shannon′s遗传多样性指数为0.348 9.与其它鱼类横向对比显示,厦门近海横带髭鲷野生群体的遗传变异处于中上水平,具有较高的遗传多样性.Hapalogenys mucronatus is a bottom associated fish generally found in warm temperate seas.It is mainly distributed in the western North Pacific Ocean,including the coasts of China,Korea,Japan and Philippines.As an excellent food fish,Hapalogenys mucronatus is highly valued in the local markets,and has a great potential for farm rearing and reproduction.To provide the genetic information of this species,the genetic diversity of 46 individuals from the wild stock of Hapalogenys mucronatus in Xiamen bay was investigated by using the inter-simple sequence repeat(ISSR) and randomly-amplified polymorphic DNA(RAPD) markers.Of the random primers screened,31 primers produced highly reproducible RAPD bands.A total of 235 discernible DNA fragments were detected,of which 106 being polymorphic.The percentage of polymorphic loci was 45.11% and Shannon′s index was 0.243 0.Of the primers screened,16 primers produced highly reproducible ISSR bands.A total of 119 discernible DNA fragments were detected,of which 76 being polymorphic.The percentage of polymorphic loci was 63.78% and Shannon′s index was 0.348 9.Compared with other fishes,the genetic diversity of H.mucronatus in Xiamen is above the middle level,with a higher genetic diversity.国家自然科学基金(30070595)资