A Globose Virion Isolated from Mud Crab Scylla serrata(Forskl) and Observation of the Pathology

从患病锯缘青蟹组织中分离出一种无囊膜球状病毒,直径50~60 nm.通过病理切片观察,病蟹肝胰腺、肌肉、心脏、肠和鳃等多处组织器官受到感染,该病毒分布在细胞浆中,呈晶格状排列,不形成包涵体;这种病毒会导致细胞瓦解,细胞核肿大变形,核质聚缩,核膜破损,肌丝断裂,线粒体坏死等症状.The cause of an unknown disease broken out among the mud crab Scylla serrata was studied.Examination by electron microscope showed that there were a number of globose viral particles in the tissue of hepatopancreas,muscle,heart,intestine and gill.The virion was approximately 50~60 nm in size,without envelope and consisted only of a symmetrical globose capsid and an electric dense core.Usually,a mass of mature virus formed a crystal structure and distributed over the cytoplasm.That virus caused some histopathological changes such as cells broken and desquamation,karyolysis or karyopyknosis,mitochondria oedema and crista broken,fascicle rupture or in disorder and so on.We also isolated the virion from the tissue of unhealthy mud crab,they had the same size and structure with the virus observed in the cells