Genetic Diversity Analysis of Reared Nibea diacanthus Stock by RAPD

采用22个随机引物对双棘黄姑鱼人工繁育群体的遗传多样性水平进行随机扩增多态DNA(RAPD)分析.共检测出248个位点,其中有104个多态位点,平均多态位点百分率P为41.9%;其个体间的平均遗传距离L为0.065 7,平均相似系数S为0.934 3,Shannon遗传多样性指数H0为0.282 9.通过与其他一些鱼类的RAPD研究结果进行对比,对双棘黄姑鱼人工繁育群体的遗传多样性水平进行了分析评估,文中同时就人工繁育及鱼类种质资源的可持续利用问题进行了初步讨论.22 random primers were used to analyze the genetic diversity of the reared Nibea diacanthus stock by using random amplified polymorphic DNA(RAPD) in this study.A total of 248 loci were amplified,of which 104 loci were polymorphic,and the mean percentage of polymorphic loci was 41.9%;the average genetic diversity(L) and the genetic similarity(S) of this stock were (0.065 7) and 0.934 3 respectively;Shannon's information index(H_0) was 0.282 9.The genetic diversity of the reared Nibea diacanthus stock was analysed and evaluated by comparing with the RAPD results of other fishes.Meanwhile,artificial propagation and sustainable utilization of fish resource were discussed in this paper.福建省自然科学基金(B0110004)资