Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis
- Santoro, Carlo
- Grattieri, Matteo
- Babanova, Sofia
- Guerrini, Edoardo
- Trasatti, Stefano Pm
- Cristiani, Pierangela
- Bestetti, Massimiliano
- Atanassov, Plamen
DOIAbstract
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. A selective oxygen biosensor based on bilirubin oxidase (BOx) was developed. The sensor was used for determining oxygen profiles in a membraneless, single-chamber microbial fuel cell (SCMFC), fed with raw wastewater. The linear response of the sensor was optimized by a diffusion layer of silica gel. A computer-controlled stage was used to obtain accurate and precise measurements. Oxygen concentration in biofilms covering electrodes was measured, showing 3mgL-1 of O2 in the bulk solution, decreasing to 0mgL-1 in the cathodic biofilm. The MFC generated power in the range of 0-0.08mW, associated to the oxygen content
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