T-DNA insertion mutagenesis

BAP Geapsi CT1 Chapitre 38International audienceThis chapter focuses on the significance of insertional mutagenesis in functional genomic studies and summarizes T-DNA mutagenesis schemes, including methodologies for creation of T-DNA mutant collections, T-DNA vectors, isolation of the tagged gene and comparisons of the frequency of mutant recovery using various strategies. Concrete examples are also given in cloning genes using T-DNA insertion lines, step by step