A multiplex lateral flow immunoassay for the rapid identification of NDM-, KPC-, IMP- and VIM-type and OXA-48-like carbapenemase-producing enterobacteriaceae
- Boutal, Hervé
- Vogel, Anais
- Bernabeu, Sandrine
- Devilliers, Karine
- Creton, Elodie
- Cotellon, Garence
- Plaisance, Marc
- Oueslati, Saoussen
- Dortet, Laurent
- Jousset, Agnès
- Simon, Stéphanie
- Naas, Thierry
- Volland, Hervé
DOIAbstract
International audienceObjectives: The global spread of carbapenemase-producing Enterobacteriaceae represents a substantial challenge in clinical practice and rapid and reliable detection of these organisms is essential. The aim of this study was to develop and validate a lateral flow immunoassay (Carba5) for the detection of the five main carbapenemases (KPC-, NDM-, VIM-and IMP-type and OXA-48-like). Methods: Carba5 was retrospectively and prospectively evaluated using 296 enterobacterial isolates from agar culture. An isolated colony was suspended in extraction buffer and then loaded on the manufactured Carba5. Results: All 185 isolates expressing a carbapenemase related to one of the Carba5 targets were correctly and unambiguously detecte...
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