Effect of different fungicides on the growth of yeast from Saccharomyces sensu stricto group

Cilj ovog istraživanja bio je utvrditi djelovanje četiri različita fungicidna pripravka na bazi djelatnih tvari mankozeb, kaptan, iprodion i zoksamid na kvasce iz skupine Saccharomyces sensu stricto u laboratorijskim uvjetima. Kvasci iz ove skupine, posebice vrste Saccharomyces cerevisiae i Saccharomyces paradoxus od iznimne su važnosti za proizvodnju vina jer svojim metabolizmom utječu na sam proces fermentacije kao i na stvaranje brojnih spojeva važnih za aromu vina. Filter-disk metodom ispitivan je utjecaj fungicidnih pripravaka koji se primjenjuju za suzbijanje bolesti vinove loze u koncentracijama preporučenima od strane proizvođača kao i u nekim umanjenim i uvećanim koncentracijama. Dokazan je utjecaj pripravaka Cadillac 80WP®, Electis WG® i Stoper® na rast ispitivanih sojeva S. cerevisiae i S. paradoxus dok Kidan® nije imao utjecaj na rast kvasaca ni pri jednoj od ispitivanih koncentracija. Najveći negativan utjecaj imao je Cadillac 80WP® koji je inhibirao rast ispitivanih sojeva čak i pri upola nižim koncentracijama od propisanih. Nije utvrđena razlika u osjetljivosti između vrsta S. cerevisiae i S. paradoxus, dok se istovremeno može zaključiti da su referentni sojevi bili osjetljiviji na Cadillac 80WP®, Electis WG® i Stoper® u usporedbi s autohtonim izolatima.The aim of this study was to determine the effect of four different fungicides based on the active substances mancozeb, captan, iprodion and zoxamide on yeasts from Saccharomyces sensu stricto groups in laboratory scale. Yeasts from these group, particulary Saccharomyces cerevisiae and Saccharomyces paradoxus species, are of great importance for wine production, because their metabolism contributes to formation of important compounds but they also affects the fermentation process itself. Filter-paper disc method was employed in order to test the effect of fungicides which are usually applied for the control of grapevine diseases at doses/concentrations recommended by the manufacturers, as well as some lower and higher concentrations. The effect of Cadillac 80WP, Electis WG and Stoper on the tested strains S. cerevisiae and S. paradox species was observed, while Kidan did not affect the growth of yeasts at any of the concentrations tested. The greatest negative impact was observed for Cadillac 80WP, which inhibited the growth of tested strains even when the applied concentrations were lower than prescribed. No differences were found between S. cerevisiae and S. paradoxus strains regarding their susceptibility to tested fungicides, while it can be concluded that the reference strains were more sensitive to Cadillac 80WP, Electis WG, and Stoper compared to indigenous isolates