The micorryzal fungi ceratobasidium sp. and sebacina vermifera promote seed germination and seedling development of the terrestrial orchid epidendrum secundum jacq

La interacción entre semillas y micorrizas hongos en germinacióny los procesos de desarrollo son complejos y están lejos de ser entendidos. La evaluación de la eficiencia de las micorrizashongos en "in vitro" semilla germinaciónde orquídeas mejorará los protocolos para fines de conservación y horticultura. Aplicando un enfoque dependiente de la cultura, aislamos 45 endofíticoshongos, de raíces de 12 orquídeaespecies, que crecen a dos niveles altitudinales en las montañas ecuatorianas. De ellos, 26 aislamientos axénicos similares a Rhizoctonia se estudiaron mediante análisis de secuencias de ADNr ribosómico nuclear, buscando micorrizas.hongos.The interaction between seeds and mycorrhizal fungi in germination and developmental processes is complex and far from being understood. The efficiency assessment of mycorrhizal fungi at “in vitro” seed germination of orchids will improve protocols for conservation and horticultural purposes. Applying a culture-dependent approach, we isolated 45 endophytic fungi, from roots of 12 orchid species, growing at two altitudinal levels in the Ecuadorian mountains. From them, 26 Rhizoctonia-like axenical isolates were studied by nuclear ribosomal rDNA sequences analysis, searching for mycorrhizal fungi . Ceratobasidium sp. and Sebacina vermifera were identified and tested for promoting germination and seedling development of Epidendrum secundum Jacq., an endangered medicinal orchid. Symbiotic cultures were stablished in Oat Meal Agar (OMA), and seeds development was compared between Murashige &