Polyhydroxyalkanoate synthesis by ralstonia eutropha from multiple substrates

M.S. University of Hawaii at Manoa 2011.Includes bibliographical references.The overall objective of this thesis is to analyze R. eutropha utilization of glucose, fructose, and LA individually and in multi-substrate cultures. There are two core objectives: 1. By using a Monod model, determine the kinetics of individual substrates. Also, compare the consumption rates of glucose and fructose in single and multiple substrate cultures, and at different C/N ratios. 2. Reveal the initial key reactions in the metabolic pathway of LA utilization by in vitro bio-conversion and HPLC analysis