Enhancing sensitivity of glucose biosensors using pulsed deposition of polypyrrole on ITO substrate

Conducting polymer based nano-biosensors has become more feasible and cost effective alternative for conventional biosensors. Sensitivity and linear range have been improved in last couple of years[1][2]. Since the effective surface area in nano-scale has a direct influence on the amount of enzyme loaded onto the biosensor, it was tried to increase the effective surface area of the polypyrrole substrate film using a unique technique. This paper describes the outcomes of the novel technique.Electro deposition of polypyrrole under a constant current is a common practice in order to fabricate a conducting polymer substrate for nano-biosensors. However, the results in Figure 1 show that the pulsed deposition of polypyrolle make a nano-structured surface