Extraction and Separation of lO-hydroxy-δ-decenoic acid from Royal Jelly by Supercritical Carbon Dioxide

本研究之目的在於萃取與分離蜂王乳中葵烯酸（10-hydroxy-δ-decenoic acid ; 10-HDA ）成分。超臨界二氧化碳（supercritical carbon dioxide ; SC-CO2 ）是一種較其它溶劑（如水、乙醇、己院、乙醚、丙酮）安全、無毒、不易燃，而且萃取物與超臨界二氧化碳很容易利用降壓方式將其分離。葵烯酸是一種抗癌、抗氧化和抗菌之物質，食用後有強烈辛辣味，使得蜂王乳不易為消費者接受。為了提高產品接受性與價值性，此研究以超臨界二氧化碳為溶劑，萃取與分離蜂王乳中葵烯酸成分做為醫學用品，與去除不悅味道後之去葵烯酸蜂王乳產品做為食品、飲料與健康食品等用途。實驗操作選擇溫度40℃，壓力10 、20 、30MPa 與添加或不添加10 ％乙醇／蜂王乳（體積/重量比）當為輔溶劑等條件，求得最適溶解度（g 葵烯酸／g CO2）、分配係數（氣相與液相之溶解度比）與選擇性（葵烯酸與蜂王乳之分配係數比）。由實驗之最佳結果，採用溫度40℃ 、壓力25MPa 、添加10％乙醇之條件下，於連續式超臨界二氧化碳萃取系統進行實驗後，顯示此條件下可去除蜂王乳中之90％葵烯酸成分。 The objective of this study was to extract and separate lO-hydroxy- -decenoic acid (10-HDA) from royal jelly. A static and a continuous extraction methods using the solvent of supercritical carbon dioxide (SC-C02) or ethanol ¡Ò SC-CO2 were designed to rearch the objective and to measure the solubility of 10-HDA in SC-CO2 or in ethanol ¡Ò SC-CO2. The extraction were performed at 40¢XC and at pressures 10, 20, and 30 MPa. Results showed distribution coefficient of 10-HDA increased with pressure. Two products, 10-HDA and non- or low 10-HDA content of royal jelly products, were obtained at 25 MPa and 40¢XC in the ethanol + SC-CO2 continuous extraction system