(60(4):263-278)Molecular Characterization of Introgression Lines from a Wild Rice, Oryza officinalis, with Resistance to Brown Planthopper

褐飛蝨為水稻的主要害蟲，除栽培稻外，野生稻為另一可提供培育抗褐飛蝨的重要基因庫。本試驗由栽培品種台農67號為輪迴親本，野生稻Oryza officinalis為貢獻親，所建立的134個導入系統 (BC2F19) 中獲得96個抗褐飛蝨系統。利用172對SSR分析134個導入系統結果顯示，抗蟲系統 (RS 5) 與感蟲系統 (RS > 5) 的O. officinalis或非親本基因型比例，平均分別為20.4%、13.0%。探討各分子標誌與褐飛蝨抗性之關聯性顯示，除第1對、第8對染色體外，其餘10對染色體上均發現與褐飛蝨抗性有關的標誌；單一系統最多具有9對染色體上含有與褐飛蝨抗性有關的標誌，但有25個抗性系統不帶有試驗中所獲得的任一個抗性連鎖標誌。另外，也發現O. officinalis及4個導入系統帶有與Bph2共連鎖KPM2的抗性標誌。所獲得的這些抗性系統及其遺傳背景資料，將有利於由O. officinalis而來的抗褐飛蝨特性在遺傳研究及育種上的利用。 Brown planthopper (BPH), Nilaparvata lugens, is one of the most destructive insect pests in rice. Species of wild rice are very important sources of germplasms for genetic improvement of cultivated rice for resistance to BPH. From the interspecific crossing of the cultivated rice variety Tainung 67 as the recurrent parent and the perennial wild rice Oryza officinalis (IRGC 100896) as the donor parent, 134 BC2F19 introgression lines (ILs) were obtained and 96 of these ILs were resistant to BPH. Results of screening of 134 ILs using 172 SSR primers showed that the average genetic ratios of resistant ILs (Resistance Score, RS 5) and susceptible ILs (RS > 5) were 20.4% and 13.0% for O. officinalis and non-parental genotypes, respectively. Markers linked to BPH resistance were detected on 10 out of the 12 rice chromosomes except for chromosomes 1 and chromosome 8 in which BPH resistant markers were absent. There was a single IL harboring BPH resistant markers on as high as 9 chromosomes, whereas 25 resistant ILs showed no markers linked to BPH resistance Furthermore, KPM2, a marker co-segregating with Bph2 gene, was also detected in the donor parent O. officinalis and 4 other ILs. The findings in this study suggest that all of the BPH resistant ILs and the information of their genetic background would be useful in further investigations of BPH resistant genes in the wild rice O. officinalis and in other rice breeding programs for improvement of resistance to BPH