We consider defects in 5d field theories corresponding to higher-rank generalizations of the ENf+1 theories; holographically dual to the Brandhuber-Oz background in type I’ String Theory. We concentrate on codimension 2 and 1 defects, corresponding, respectively, to 3d and 4d defect Quantum Field Theories. We study holographically such defect theories by considering supersymmetric probe D4- and D6-branes in the AdS6, whose fluctuations allow us to study the spectrum of mesonic operators of the defect theories. In the case of D4-branes, we also consider wrappings in the internal space which can be regarded as generalizations of the configurations capturing the antisymmetric Wilson loopJ. M. P. and A. V. R. are funded by the Spanish grant FP...