Rapid LA-REIMS and comprehensive UHPLC-HRMS for metabolic phenotyping of feces
- Van Meulebroek, Lieven
- Cameron, Simon
- Plekhova, Vera
- De Spiegeleer, Margot
- Wijnant, Kathleen
- Michels, Nathalie
- De Henauw, Stefaan
- Lapauw, Bruno
- Takats, Zoltan
- Vanhaecke, Lynn
DOIAbstract
Ambient ionization-based techniques hold great potential for rapid point-of-care applicable metabolic fingerprinting of tissue and fluids. Hereby, feces represents a unique biospecimen as it integrates the complex interactions between the diet, gut microbiome and host, and is therefore ideally suited to study the involvement of the diet-gut microbiome axis in metabolic diseases and their treatments at a molecular level. We present a new method for rapid (<10 s) metabolic fingerprinting of feces, i.e. laser-assisted rapid evaporative ionization mass spectrometry (LA-REIMS) with an Nd:YAG laser (2940 nm) and quadrupole Time-of-Flight mass spectrometer as main components. The LA-REIMS method was implemented on mimicked crude feces samples from...
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