Value of Argyrophilic Nucleolar Organizing Region Protein Determinations in Nondiagnostic Fine Needle Aspiration Samples (Due to Insufficient Cell Groups) of Thyroid Nodules

WOS: 000326422900007PubMed: 24341126OBJECTIVE: To evaluate the diagnostic value of argyrophilic nucleolar organizing regions (AgNORs) in the nondiagnostic fine needle aspiration biopsy (NFNAB) specimens (due to insufficient cell groups) of cases with thyroid nodules. STUDY DESIGN: Thirty-five patients with NFNAB were included in the study. FNAB stained with Giemsa was faded using the McKee technique, then stained for AgNOR detection according to a specific protocol. One hundred nuclei per individual were evaluated to detect the AgNOR count/nucleus and total AgNOR area/nuclear area (TNA/NA) of individual cells by using a computer program. RESULTS: The AgNOR values of patients with NFNAB were between 1.2-2.4% for AgNOR count/nucleus and between 3.1-9.1% for TNA/NA. When the cutoff values were taken as >3 for AgNOR count/nucleus and >8 for TNA/NA, the sensitivity ratios were 100% and 97% for AgNOR count/nucleus and TNA/NA for discriminating benign/malignant lesions. CONCLUSION: By using some cutoff values obtained from a modified AgNOR staining method, benign/malignant potential of thyroid aspirations with NFNAB may be estimated