DETERMINATION OF THE PRESENCE AND ANTIBIOTIC SUSCEPTIBILITY OF FRANCISELLA TULARENSIS IN SPRING WATERS IN DUZCE, TURKEY

Dulger, Basaran/0000-0002-3184-2652WOS: 000409399600025This study aimed to investigate the presence of Francisella tularensis in the spring waters of Duzce. Water samples were collected at regular monthly intervals from 20 different stations between November 2015 and November 2016. Membrane filtration method was used for water sample filtration. In a culture study, glucose cysteine blood agar selective medium was used for F. tularensis isolation. The Tu14 primer, specific to F. tularensis, was used in PCR for molecular identification. F. tularensis was isolated from 2 of the 240 water samples. Antibiotic susceptibility of F. tularensis was determined by disk diffusion method using doxycycline, erythromycin, streptomycin, ciprofloxacin, chloramphenicol, gentamicin, meropenem, tobramycin, tetracycline, nitrofurantoin and imipenem antibiotics.The highest efficacy was demonstrated by Ciprofloxacin.Duzce UniversityDuzce University [BAP-2014.04.01.286]This work was supported by the Research Fund of Duzce University. (Project number BAP-2014.04.01.286)