The sensitisation of TRPV1 mediated responses by Prostaglandin and bradykinin and the signalling pathways involved

TRPV1 ion channels have crucial roles in inflammatory hyperalgesia as TRPV1-/- mice show reduced thermal hyperalgesia response in response to tissue inflammation. The activity of TRPV1 is upregulated in the case of inflammation due to the release of different inflammatory mediators which can modulate the function of TRPV1. Bradykinin is an algogenic substance which is released at the site of inflammation and it’s known to produce thermal hyperalgesia. However, the mechanism underlying the sensitising effect of bradykinin has not been full elucidated. The aim of present project is to investigate how bradykinin mediates the sensitisation of capsaicin evoked calcium responses in DRG neurons. With the use of immunocytochemical and morphometric techniques, the present study has demonstrated the expression and distribution of EP4, TRPV1, COX-1 and B2 proteins mainly in small diameter (