Isolation and examination of a bacteriophage of a pathogenic strain of Burkholderia pseudomallei

Information regarding the genomes of bacteriophage of Burkholderia pseudomallei is still limited with few genomes sequenced. Other bacterial species have been proved to have lysogenic bacteriophage that play a clear role in virulence, but this is not the case with B. pseudomallei (Summer et. al., 2007) and further investigation is warranted. In this work, a bacteriophage was isolated from a highly virulent strain of B. pseudomallei The bacteriophage caused alterations in colonial morphology of other B. pseudomallei isolates, but did not clear changes in virulence in a BALB/c mouse model. Examination of the majority of the phage genome was then carried out