Root colonizing and soil borne communities of arbuscular mycorrhizal fungi differ among soybean fields with contrasting historical land use

Arbuscular mycorrhizal fungi (AMF) are a key component of soil microbiota in natural and anthropogenic ecosystems. Even though soil type and climate conditioned land uses in the past, soybean cultivation has overrode such limitations and replaced the earlier diverse agro- and natural ecosystems in many countries of South America. We investigated whether actual diversity patterns of local AMF communities were determined by previous land uses and their intrinsic environmental conditions. We sequenced AMF DNA from root and soil samples collected from current soybean fields with three historical land use situations (HLU): agricultural fields, livestock farming and forest sites. We detected overall high AMF richness: 87 virtual taxa (VT) in soil and 69 VT in soybean roots. Mean number of VT per sample ranged from 8.1 to 19.2; it was not affected by HLU nor type of sample, but correlated with soil texture, pH, and plant density. Conversely, AMF community composition did significantly diverge among HLU and type of sample. A distinctive community composition was observed in roots of historical agricultural fields which differed from any other soil and root sample evaluated in this study. We attribute this finding to variations in the abundance pattern of predominant AMF taxa (Glomeraceae and Gigasporaceae). Our results indicate that soybean cultivation supports relatively high AMF diversity, with apparent legacies from earlier management and natural habitats in the composition of resident AMF communities.EEA Marcos JuárezFil: Faggioli, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Cabello, Marta Noemí. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires; ArgentinaFil: Grilli, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Vasar, Martti. University of Tartu. Department of Botany; EstoniaFil: Covacevich, Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; ArgentinaFil: Öpik, Maarja. University of Tartu. Department of Botany; Estoni