In-Vitro Mechanisms Of Cell Proliferation Induction: A Novel Bioactive Treatment For Accelerating Wound Healing

These studies tested the effects of a novel biophysical stimulus, cell proliferation induction (CPI), a low-level, confined, radiofrequency signal, on the rate of cell proliferation in vitro. A series of experiments were conducted to establish the dose and time course parameters of the CPI signal effects on fibroblast and epithelial cell proliferation. Subsequent studies examined the potential requirement for specific biochemical signaling pathways in the CPI signal\u27s pro-proliferative effect. The results showed that CPI treatment significantly increased the proliferation of both fibroblasts and epithelial cells as a function of dose and time. In addition, CPI treatment caused an immediate release of a diffusable growth factor via a Ca+2 dependent pathway. The nature of these findings suggests that CPI serves as a cellular mitogen, which signals rapid induction into the cell cycle. Therefore, this novel bioactive treatment that uses a specific form of radiofrequency signal may have the potential to effectively accelerate wound closure via stimulation of endogenous growth factor pathways with subsequent proliferation of cell types critical to the wound reconstruction process