Evidence of the absence of Human African Trypanosomiasis in two Northern Districts of Uganda: analyses of cattle, pigs and tsetse flies for the presence of Trypanosoma brucei gambiense
- Cunningham, Lucas
- Lingley, Jessica
- Tirados, Inaki
- Esterhuizen, Johan
- Opiyo, Mercy
- Mangwiro, Clement
- Lehane, Mike
- Torr, Steve
DOIAbstract
Background Large-scale control of sleeping sickness has led to a decline in the number of cases of Gambian human African trypanosomiasis (g-HAT) to <2000/year. However, achieving complete and lasting interruption of transmission may be difficult because animals may act as reservoir hosts for T. b. gambiense. Our study aims to update our understanding of T. b. gambiense in local vectors and domestic animals of N.W. Uganda. Methods We collected blood from 2896 cattle and 400 pigs and In addition, 6664 tsetse underwent microscopical examination for the presence of trypanosomes. Trypanosoma species were identified in tsetse from a subsample of 2184 using PCR. Primers specific for T. brucei s.l. and for T. brucei sub-species were used to s...
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