MOLECULAR DIAGNOSTIC OF NEISSERIAE GONORRHOEAE IN THE TEACHING INSTITUTE OF PUBLIC HEALTH PRIMORSKO-GORANSKA COUNTRY

Infekcije Neisseria gonorrhoeae jedne su od najučestalijih spolno prenosivih infekcija u svijetu, a nedijagnosticirane i neliječene infekcije povezane su s upalnom bolesti zdjelice koja može dovesti do neplodnosti. Infekcija N.gonorrhoeae također je povezana s olakšanim prijenosom ostalih spolno prenosivih bolesti (HIV, Chlamydia trachomatis). N.gonorrhoeae je gram negativni diplokok koji svojim površinskim strukturama penetrira u mukozu i preživljava unutar polimorfonukleara te uzrokuje upalu uretre. Cilj ovog rada je prikazati metodu molekularne dijagnostike N.gonorrhoeae koja se koristi u Odsjeku za molekularnu dijagnostiku Nastavnog Zavoda za javno zdravstvo Primorsko-goranske županije te analizom rezultata provedenih dijagnostičkih postupaka u razdoblju od 2014.-2016. godine prikazati učestalost infekcija uzrokovanih N.gonorrhoeae. Svi zaprimljeni uzorci u ispitivanom razdoblju od 2014.-2016. godine obrađeni su sukladno radnim uputama Odsjeka za molekularnu dijagnostiku (izolacija DNK, RT-PCR). Za statističku obradu podataka korišteni su podaci navedenog odsjeka. U razdoblju od 2014.-2016. godine ukupno je obrađeno 2684 uzoraka, od kojih je 2189 (81,56%) dobiveno od žena, a 495 (18,44%) od muškaraca. N.gonorrhoeae je dokazana u 24 (0,89%) uzorka, a 2660 (99,11%) uzoraka je negativno.Infections caused by Neisseria gonorrhoeae are one of the most common sexually transmitted infections in the world, undiagnosed and untreated infections are linked to Pelvic inflammatory disease (PID) which can lead to infertility. Infection caused by N.gonorrhoeae is also linked to easier transmission of other sexually transmitted diseases (HIV, Chlamydia trachomatis). N.gonorrhoeae is gram negative diplococcus which is using its superficial structures to penetrate the mucosa, it is able to survive inside polymorphonuclear cells and causes the inflammation of the urethra. The aim of this paper is to present the method of molecular diagnostics of N.gonorrhoeae which is used in in the Section for Molecular Diagnostics of the Teaching Institute of public health of the Primorsko-Goranska County and analyzing results of diagnostic procedures conducted in the period of 2014-2016 to present frequency of infections caused by N.gonorrhoeae. All samples received during the trial period of 2014-2016 are processed in accordance with the operating instructions of the Section of Molecular Diagnostics (DNA isolation, RT-PCR). Data from the same section was used for statistical data processing. During the period of 2014-2016 in total 2684 samples were processed, of which 2189 (81,56%) were obtained from women, and 495 (18,44%) were obtained from men. N.gonorrhoeae was detected in 24 (0,89%) samples, 2660 (99,11%) samples were negative