International audienceVirology has greatly benefited from the introduction of fluorescent proteins (FP's) as tags to viral as well as cellular structures. With advanced imaging technologies it is now possible to observe host-pathogen interactions in living cell systems in real-time. The generation of high-quality genetic tools to study host-pathogen interactions therefore becomes imperative for the further development of this type of analysis. In this chapter we describe a universal and reliable method to rapidly generate stable cell lines expressing FP-tagged proteins to be used for the analysis of host-pathogen interactions. The protocol is exemplified for two cellular structures recognized for their importance in the host-pathogen interp...
<p>The use of fluorescent proteins, particularly when genetically fused to proteins of biological in...
Genetically identical cells growing under homogeneous growth conditions often display cell-cell vari...
The process for transfection of cells with expression and gene-trap vectors expressing fluo-rescent ...
Fluorescent protein fusions (FPFs) have been used to address a wide range of questions in individual...
<div><p>Deciphering a function of a given protein requires investigating various biological aspects....
Deciphering a function of a given protein requires investigating various biological aspects. Usually...
This protocol provides a step-by-step method to create recombinant fluorescent fusion proteins that ...
This protocol provides a step-by-step method to create recombinant fluorescent fusion proteins that ...
Many longstanding questions about dynamics of virus-cell interactions can be answered by combining f...
Coupling the genetic code expansion technique with bioorthogonal reactions enables precise control o...
10 SUMMARY Proteins represent one of the basic building blocks of all organisms. To understand their...
Traditionally, cell line generation requires several months and involves screening of over several h...
Traditionally, cell line generation requires several months and involves screening of over several h...
We present a high-content screen (HCS) for the simultaneous analysis of multiple phenotypes in HeLa ...
<div><p>Traditionally, cell line generation requires several months and involves screening of over s...
<p>The use of fluorescent proteins, particularly when genetically fused to proteins of biological in...
Genetically identical cells growing under homogeneous growth conditions often display cell-cell vari...
The process for transfection of cells with expression and gene-trap vectors expressing fluo-rescent ...
Fluorescent protein fusions (FPFs) have been used to address a wide range of questions in individual...
<div><p>Deciphering a function of a given protein requires investigating various biological aspects....
Deciphering a function of a given protein requires investigating various biological aspects. Usually...
This protocol provides a step-by-step method to create recombinant fluorescent fusion proteins that ...
This protocol provides a step-by-step method to create recombinant fluorescent fusion proteins that ...
Many longstanding questions about dynamics of virus-cell interactions can be answered by combining f...
Coupling the genetic code expansion technique with bioorthogonal reactions enables precise control o...
10 SUMMARY Proteins represent one of the basic building blocks of all organisms. To understand their...
Traditionally, cell line generation requires several months and involves screening of over several h...
Traditionally, cell line generation requires several months and involves screening of over several h...
We present a high-content screen (HCS) for the simultaneous analysis of multiple phenotypes in HeLa ...
<div><p>Traditionally, cell line generation requires several months and involves screening of over s...
<p>The use of fluorescent proteins, particularly when genetically fused to proteins of biological in...
Genetically identical cells growing under homogeneous growth conditions often display cell-cell vari...
The process for transfection of cells with expression and gene-trap vectors expressing fluo-rescent ...