Comparative structural and catalytic properties of oxygen-evolving photosystem II preparations

Biochemical techniques now exist to produce the oxygen-evolving complex of photosystem II (PSII) and its associated photochemical redox reactions in various states of purity. These preparations permit one to assess the structural roles of polypeptides in promoting activity by using selective extraction techniques which remove certain polypeptides, to carry out reconstitution studies which re-establish activity, and, in the case of more recently developed, highly purified preparations discussed in this overview, to identify the minimal polypeptide complement necessary for photosynthetic oxygen evolution activity. These comparative investigations also suggest a tentative structure for an oxygen-evolving PSII core complex whose primary constituents are a hydrophobic complex of polypeptide, manganese, calcium and chloride, and the 33 kDa extrinsic polypeptide.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/43533/1/11120_2004_Article_BF00032704.pd