Autoradiography with acrylamide gel slab electrophoresis

A method for electrophoresis and autoradiography with a multisample gel slab system has been described. The method obviates slicing of the gel, retains the high degree of resolution of bands in the autoradiogram, and enables comparison of protein and radioactivity patterns of many samples under essentially identical conditions. Within a limited range, a linear relation is obtained between radioactivity and optical density in the autoradiogram, as well as between protein concentration and optical density in the gel.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/32986/1/0000370.pd