Lipoatrophic diabetes: An improved procedure for the isolation and purification of a diabetogenic polypeptide from urine

An improved procedure is outlined for the isolation of a diabetogenic polypeptide from urine of patients with lipoatrophic diabetes. The compound provokes insulin resistance and temporary loss of glucose tolerance when administered to dogs and humans. It is now free of a toxic contaminant which, by the previous method, had to be removed by more drastic treatment of the compound than was deemed suitable. Its physicochemical properties closely resemble those of a diabetogenic polypeptide isolated from the adenohypophyses of beef, sheep, and hog described previously from this laboratory.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/32943/1/0000326.pd