Heterogeneity of tissue dehydrogenases

Rat tissue homogenates have been subjected to zone electrophoresis in starch gel. Lactate, malate, isocitrate, glucose 6-phosphate and [alpha]-glycerophosphate dehydrogenase activity was directly visualized by incubating the gel strips in solutions containing a specific substrate, a tetrazolium salt, methylene blue and the appropriate pyridine nucleotide. Electrophoretic heterogeneity of some of the dehydrogenases was observed in material from different organs. A similar multiplicity of components in some specific dehydrogenases was also demonstrated in cytologically homogeneous material including cells grown by tissue culture techniques. Significance of this multiplicity of enzymes is discussed.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/32402/1/0000477.pd