The chemical synthesis of 2-keto-3-deoxy-octonate and its cleavage by a specific aldolase

We have previously reported (Heath and Ghalambor, 1963) the occurrence of 2-keto-3-deoxy-octonate (KDO) as a glycosidically-bound constituent of cell wall lipopolysaccharide (LPS) of 0111-B4 and the enzymatic synthesis of CMP-KDO (Ghalambor and Heath, 1963). This report presents evidence for the chemical synthesis of KDO and its enzymatic cleavage (KDO-aldolase) to yield equimolar quantities of pyruvate and D-arabinose.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/32223/1/0000283.pd