1. 1. Previous studies have demonstrated the presence of glutathione S-transferases in the skin of rodents and humans. This study represents the first attempt to purify cytosolic glutathione S-transferases from skin of 3-day-old rats.2. 2. A partial purification of the enzyme was achieved by a two-step procedure: affinity chromatography followed by HPLC. Two peaks, one major (P-1) and one minor (P-2), were resolved by HPLC containing about 82% and 10% of the recovered activity, respectively.3. 3. The major form exhibited an overall purification of about 2270-fold with a specific activity of about 73 [mu]moles/min/mg protein towards 1-chloro-2,4-dinitrobenzene.4. 4. The kinetic data for P-1 yielded mean Km values of 2.39 mM for 1-chloro-2,4-...
A simple High Performance Liquid Chromatography procedure is detailed for the purification of Glutat...
The interaction among chemicals listed in the Directive CE/39/2000 with skin notation and glutathion...
AbstractGlutathione metabolism was studied in rat liver during diethylnitrosamine (DEN) carcinogenes...
The glutathione S-transferases (GST) are a family of widely distributed multifunctional detoxificati...
AbstractA simple small-scale purification procedure is described for GSH transferase E. This enzyme ...
Analysis of the glutathione S-transferase activity from the hepatic cytosol of untreated, 3-me thylc...
1. The major hepatic glutathione S-transferases (GSTs) from gerbil, guinea-pig, hamster, mouse and r...
A novel hepatic enzyme, glutathione S-transferase K, is described that, unlike previously characteri...
This study has examined the expression of the glutathione S-transferases (GSH S-T) in fetal rat live...
In this study the purification of human basic and near-neutral liver, and human basic and acidic lun...
A new isozyme of Glutathione-S-transferase (GST) with a more acidic pI (6.7) than other forms of GST...
We conducted a comprehensive characterization of the ontogeny of glutathione (GSH) and its related e...
Microsomal glutathione transferase (MGST) is a membrane bound detoxification enzyme, which has been ...
A simple High Performance Liquid Chromatography procedure is detailed for the purification of Glutat...
The glutathione S-transferases are a group of enzymes which catalyse the conjugation of reduced glut...
A simple High Performance Liquid Chromatography procedure is detailed for the purification of Glutat...
The interaction among chemicals listed in the Directive CE/39/2000 with skin notation and glutathion...
AbstractGlutathione metabolism was studied in rat liver during diethylnitrosamine (DEN) carcinogenes...
The glutathione S-transferases (GST) are a family of widely distributed multifunctional detoxificati...
AbstractA simple small-scale purification procedure is described for GSH transferase E. This enzyme ...
Analysis of the glutathione S-transferase activity from the hepatic cytosol of untreated, 3-me thylc...
1. The major hepatic glutathione S-transferases (GSTs) from gerbil, guinea-pig, hamster, mouse and r...
A novel hepatic enzyme, glutathione S-transferase K, is described that, unlike previously characteri...
This study has examined the expression of the glutathione S-transferases (GSH S-T) in fetal rat live...
In this study the purification of human basic and near-neutral liver, and human basic and acidic lun...
A new isozyme of Glutathione-S-transferase (GST) with a more acidic pI (6.7) than other forms of GST...
We conducted a comprehensive characterization of the ontogeny of glutathione (GSH) and its related e...
Microsomal glutathione transferase (MGST) is a membrane bound detoxification enzyme, which has been ...
A simple High Performance Liquid Chromatography procedure is detailed for the purification of Glutat...
The glutathione S-transferases are a group of enzymes which catalyse the conjugation of reduced glut...
A simple High Performance Liquid Chromatography procedure is detailed for the purification of Glutat...
The interaction among chemicals listed in the Directive CE/39/2000 with skin notation and glutathion...
AbstractGlutathione metabolism was studied in rat liver during diethylnitrosamine (DEN) carcinogenes...