Tyrosine hydroxylase immunoreactivity identifies possible catecholaminergic fibers in the organ of Corti

Antibodies to tyrosine hydroxylase. dopamine [beta]-hydroxylase and phenylethanolamine N-methyltransferase were used in an immunocytochemical examination of catecholamines in the cochlea. In cryostat sections, tyrosine hydroxylase and dopamine [beta]-hydroxylase-like immunoreactivities fibers were seen in the modiolus that did not extend to the organ of Corti. These corresponded to blood vessel-associated and non-blood vessel-associated fibers that have been previously described with histofluorescence. In surface preparations, tyrosine hydroxylase-like immunoreactivity was seen in the organ of Corti. in the inner and tunnel spiral bundles, suggesting an efferent component may be catecholaminergic.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/26856/1/0000421.pd