A specific Enzyme-Linked Immunosorbent Assay (ELISA) for the determination of human C5a antigen
- Kunkel, Steven L.
- Manderino, George L.
- Marasco, Wayne A.
- Kaercher, Kip
- Hirata, Arthur A.
- Ward, Peter A.
Publication date
September 1983
DOI Publisher
Elsevier BV ISSN
0022-1759 Citation count (estimate)
5Abstract
An enzyme-linked immunosorbent assay has been developed to detect human C5a antigen. This ELISA methodology has been shown to be a highly sensitive technique capable of detecting C5a antigen concentrations below 10 ng/ml. The microELISA technique used in this study is specific for human C5a and C5a des arg (C5a antigen) but not for human C5. Conditions to establish sensitivity and specificity are outlined in this report.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/25112/1/0000545.pd
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