Mass spectrometry(MS) have become an increasingly popular analysis method for high throughput experiments on proteins in biology. SILAC(stable isotope labeling by amino acids in cell culture) is a method within MS that uses labels to differentiate between the same protein from two cell cultures(protein pairs) of the same organism. To identify the protein pairs in SILAC MS/MS is used. The current/automatic method for MS/MS uses the most intense peaks from each single MS for the MS/MS analysis. This often result in incomplete pairs, and proteins that have a low abundance gets ignored. We created a program able to identify these pairs, and the researcher can use the data from it as an inclusion list for the MS/MS run. The program should be eas...
Mass spectrometry (MS) is used in peptidomics to find novel endogenous peptides that may lead to the...
Stable incorporation of labeled amino acids in cell culture is a simple approach to label proteins i...
AbstractSystematic analysis of the function of genes can take place at the oligonucleotide or protei...
Precise quantification is a major issue in contemporary proteomics. Both stable-isotope-labeling and...
Metabolic stable isotope labeling is increasingly employed for accurate protein (and metabolite) qua...
Analysis by combined liquid chromatographic separation and mass spectrometry (LC-MS) is rapidly beco...
Background Proteomics is the study of the proteome, and is critical to the understan...
Pull-downs based on tag fusion proteins as well as immunoprecipitations (IP) are widely used methods...
The reliable identification of protein interaction partners and how such interactions change in resp...
Stable isotope labelling by amino acids in cell culture (SILAC) is a technique that allows proteomic...
Mass spectrometry (MS), in its various forms, has become an essential tool for genome and proteome a...
<p>(A) Determination of incorporation efficiency by MALDI-TOF/TOF-MS. Peaks annotated as R0 (left), ...
In recent years, mass spectrometry has become a staple technique in biochemistry and molecular biolo...
Despite progress in the characterization of their genomes, proteomes of several model organisms are ...
Metabolic labeling techniques have recently become popular tools for the quantitative profiling of p...
Mass spectrometry (MS) is used in peptidomics to find novel endogenous peptides that may lead to the...
Stable incorporation of labeled amino acids in cell culture is a simple approach to label proteins i...
AbstractSystematic analysis of the function of genes can take place at the oligonucleotide or protei...
Precise quantification is a major issue in contemporary proteomics. Both stable-isotope-labeling and...
Metabolic stable isotope labeling is increasingly employed for accurate protein (and metabolite) qua...
Analysis by combined liquid chromatographic separation and mass spectrometry (LC-MS) is rapidly beco...
Background Proteomics is the study of the proteome, and is critical to the understan...
Pull-downs based on tag fusion proteins as well as immunoprecipitations (IP) are widely used methods...
The reliable identification of protein interaction partners and how such interactions change in resp...
Stable isotope labelling by amino acids in cell culture (SILAC) is a technique that allows proteomic...
Mass spectrometry (MS), in its various forms, has become an essential tool for genome and proteome a...
<p>(A) Determination of incorporation efficiency by MALDI-TOF/TOF-MS. Peaks annotated as R0 (left), ...
In recent years, mass spectrometry has become a staple technique in biochemistry and molecular biolo...
Despite progress in the characterization of their genomes, proteomes of several model organisms are ...
Metabolic labeling techniques have recently become popular tools for the quantitative profiling of p...
Mass spectrometry (MS) is used in peptidomics to find novel endogenous peptides that may lead to the...
Stable incorporation of labeled amino acids in cell culture is a simple approach to label proteins i...
AbstractSystematic analysis of the function of genes can take place at the oligonucleotide or protei...