Two Dimensional Liquid Chromatographic Separations of Plants Extracts

In this study, the potential of using off-line or online two dimensional liquid chromatography (2D LC) separations of plant extracts was examined. A new approach to high speed, online isocratic elution comprehensive 2D LC based on the use of hydrophilic interaction liquid chromatography (HILIC) chromatography in the first dimension, and a fast second dimension utilizing high temperature and Hypercarb porous carbon stationary phase is described. Entirely conventional isocratic elution instrumentation and columns were assembled in a system which could separate the complex mixture of compounds extracted from rare Serbian plants. The second dimension column was used at high flow rate in isocratic mode and a high column temperature of 110°C. Fractions eluted from the first dimension HILIC column were subjected to the second dimension either online (using an interface) or off-line. The online HILIC-Hypercarb combination demonstrated large peak capacities as compared to other modes