In eukaryotes the entire genome is replicated precisely once in each cell cycle. No DNA is re-replicated until passage through mitosis into the next S-phase. We have used a cell-free DNA replication system from Xenopus eggs to determine which mitotic changes permit DNA to re-replicate. The system efficiently replicates sperm chromatin, but no DNA is re-replicated in a single incubation. This letter shows that nuclei replicated in vitro are unable to re-replicate in fresh replication extract until they have passed through mitosis. However, the only mitotic change which is required to permit re-replication is nuclear envelope permeabilization. This suggests a simple model for the control of DNA replication in the cell cycle, whereby an essent...
In eukaryotes, the initiation of DNA synthesis requires the assembly of a pre-replicative complex (p...
Cell-free systems from eggs of the frog Xenopus laevis are able to perform most of the acts of eukar...
The Xenopus cell free system has proved a good model system to study in vitro DNA replication and th...
In eukaryotes the entire genome is replicated precisely once in each cell cycle. No DNA is re-replic...
We have developed a cell-free system from frog eggs that efficiently initiates and completes a singl...
Abstract. Xenopus egg extracts treated with the pro-tein kinase inhibitor 6-dimethylaminopurine (6-D...
Aspects of the regulation of DNA replication and mitosis have been studied using a cell-free extract...
Xenopus egg extracts treated with the protein kinase inhibitor 6-dimethylaminopurine (6-DMAP) are un...
Purified DNA undergoes a single round of semicon-servative replication when incubated in extracts of...
In this article, we study how intercalation-induced changes in chromatin and DNA topology affect chr...
In eukaryotes, the initiation of DNA synthesis requires the assembly of a pre-replicative complex (p...
AbstractBackground: A complex of MCM proteins is implicated in ensuring that DNA replicates only onc...
In eukaryotes, the initiation of DNA synthesis requires the assembly of a pre-replicative complex (p...
Cell-free systems from eggs of the frog Xenopus laevis are able to perform most of the acts of eukar...
The Xenopus cell free system has proved a good model system to study in vitro DNA replication and th...
In eukaryotes the entire genome is replicated precisely once in each cell cycle. No DNA is re-replic...
We have developed a cell-free system from frog eggs that efficiently initiates and completes a singl...
Abstract. Xenopus egg extracts treated with the pro-tein kinase inhibitor 6-dimethylaminopurine (6-D...
Aspects of the regulation of DNA replication and mitosis have been studied using a cell-free extract...
Xenopus egg extracts treated with the protein kinase inhibitor 6-dimethylaminopurine (6-DMAP) are un...
Purified DNA undergoes a single round of semicon-servative replication when incubated in extracts of...
In this article, we study how intercalation-induced changes in chromatin and DNA topology affect chr...
In eukaryotes, the initiation of DNA synthesis requires the assembly of a pre-replicative complex (p...
AbstractBackground: A complex of MCM proteins is implicated in ensuring that DNA replicates only onc...
In eukaryotes, the initiation of DNA synthesis requires the assembly of a pre-replicative complex (p...
Cell-free systems from eggs of the frog Xenopus laevis are able to perform most of the acts of eukar...
The Xenopus cell free system has proved a good model system to study in vitro DNA replication and th...