Current advancements in biological protein discovery utilize bi-partite methods of fluorescence detection where chromophore and scaffold are uncoupled. One such technology, called fluorogen-activating proteins (FAPs), consists of single-chain-variable-fragments (scFvs) selected against small organic molecules (fluorogens) that are non-fluorescent in solution, but highly fluorescent when bound to the scFv. In unusual circumstances a scFv may activate similar fluorogens from a single chemical family. In this report we identified a scFv biosensor with fluorescence activity against multiple fluorogens from two structurally dissimilar families. In-vitro analysis revealed highly selective scFv-ligand interactions at sub-micromolar ranges. Additio...
<p>CXCR4 is expressed as a fusion protein with α-FAP and US27 is expressed as a fusion protein with ...
Diversity-oriented fluorescence library approaches have significantly accelerated the development of...
Monitoring the trafficking of multiple proteins simultaneously in live cells is of great interest be...
<p>Current advancements in biological protein discovery utilize bi-partite methods of fluorescence d...
Imaging of live cells has been revolutionized by genetically encoded fluorescent probes, most famous...
<p>Fluorogen-activating-proteins (FAPs) are a novel platform of fluorescence biosensors utilized for...
<p>Fluorescence biosensors are indispensable tools for understanding protein behavior and function i...
Fluorescence biosensors are indispensable tools for understanding protein behavior and function in c...
Fluorescence imaging is a powerful technique for the real-time noninvasive monitoring of protein dyn...
<p>Novel fluorescent tools such as green fluorescent protein analogues and fluorogen activating prot...
Fluorescent probes constitute a valuable toolbox to address a variety of biological questions and th...
Novel fluorescent tools such as green fluorescent protein analogues and fluorogen activating protein...
Through the efforts of many groups, a wide range of fluorescent protein reporters and sensors based ...
Through the efforts of many groups, a wide range of fluorescent protein reporters and sensors based ...
A versatile platform for the one-step fluorescence detection of both monovalent and multivalent prot...
<p>CXCR4 is expressed as a fusion protein with α-FAP and US27 is expressed as a fusion protein with ...
Diversity-oriented fluorescence library approaches have significantly accelerated the development of...
Monitoring the trafficking of multiple proteins simultaneously in live cells is of great interest be...
<p>Current advancements in biological protein discovery utilize bi-partite methods of fluorescence d...
Imaging of live cells has been revolutionized by genetically encoded fluorescent probes, most famous...
<p>Fluorogen-activating-proteins (FAPs) are a novel platform of fluorescence biosensors utilized for...
<p>Fluorescence biosensors are indispensable tools for understanding protein behavior and function i...
Fluorescence biosensors are indispensable tools for understanding protein behavior and function in c...
Fluorescence imaging is a powerful technique for the real-time noninvasive monitoring of protein dyn...
<p>Novel fluorescent tools such as green fluorescent protein analogues and fluorogen activating prot...
Fluorescent probes constitute a valuable toolbox to address a variety of biological questions and th...
Novel fluorescent tools such as green fluorescent protein analogues and fluorogen activating protein...
Through the efforts of many groups, a wide range of fluorescent protein reporters and sensors based ...
Through the efforts of many groups, a wide range of fluorescent protein reporters and sensors based ...
A versatile platform for the one-step fluorescence detection of both monovalent and multivalent prot...
<p>CXCR4 is expressed as a fusion protein with α-FAP and US27 is expressed as a fusion protein with ...
Diversity-oriented fluorescence library approaches have significantly accelerated the development of...
Monitoring the trafficking of multiple proteins simultaneously in live cells is of great interest be...